For the production fo glutathione by enzymatic method, Υ-glutamylcysteine synthetase and glutathione synthetase were purified from genetically engineered Escherichia coli cells Glutathione is biosynthesized by the consecutive actions
ofΥ-glutamylcysteine synthetase and glutathione synthetase via ATP-requiring reaction. To eliminate feedback inhibition ofΥ-glutamylcysteine synthetase by glutathione, Υ-glutamylcysteine synthetase and glutathione synthetase were perfonned
seperately. The conditions for the production of glutathione and Υ-glutamylcysteine are 25mM MgCl2, 30mM potassium phosphate, 38℃, pH7.6. Under the these con dition, 18.2mM of glutathione was obtained and the conversion ratio was 92%.
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